Lymphocytes blast-transformation reaction: modification for allergological practice
https://doi.org/10.17650/1726-9784-2018-17-2-88-92
Abstract
Introduction. Lymphocytes blast-transformation reaction (RBLT) is necessary for patient’s inspection with immunologic infringements. It is applied in the different fields of medicine to identification of a sensitization to antigens (allergens).
Research objective – to modify and automate RBTL for application in routine laboratory practice.
Materials and methods. RBTL, antigens (allergens). Software: Windows 7; Intel Pentium G4500 CPU. Digital CMOS video camera, Mikmed-6 microscope.
Results. We analyzed difficulties when using RBTL in laboratory allergological practice. We created program files for automation of RBTL.
Conclusion. We adapted and automated RBTL for laboratory diagnostics using.
About the Authors
I. V. Manina
Institute of Allergology and Clinical Immunology
Russian Federation
Russian Federation
Bldg 1, 20 Malaya Bronnaya St., Moscow 123104
V. Yu. Sergeev
Institute of Allergology and Clinical Immunology
Russian Federation
Russian Federation
Bldg 1, 20 Malaya Bronnaya St., Moscow 123104
N. V. Golubtsova
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Russian Federation
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
A. Yu. Sergeev
I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Secheniv University)
Russian Federation
Russian Federation
Bldg 4, 2 Bol’shaya Pirogovskaya St., Moscow 119991
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Review
For citations:
Manina I.V., Sergeev V.Yu., Golubtsova N.V., Sergeev A.Yu. Lymphocytes blast-transformation reaction: modification for allergological practice. Russian Journal of Biotherapy. 2018;17(2):88-92. (In Russ.) https://doi.org/10.17650/1726-9784-2018-17-2-88-92
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