LFA-1, MAC-1 LEUKOCITE INTEGRINS – TARGET FOR TUMOUR IMMUNITY MAGNIFICATION

The aim of this investigation was to evaluate the expression of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) β₂ integrins on blood cells in CBA mice in ontogenesis as well as under the dry extract of multifitoadaptogen administration during the first month of postnatal development.

Materials and methods. The study was carried out on CBA male mice (CBA/LacY). The experimental mice received 0.3 % dry multiphytoadaptogene extract water-solution during the first month of life (preventive administration). Preparation was produced by a desiccation technology of forty medical herbs (including Panax ginseng, Eleutherococcus senticosus, Rhodiola rosea) water-ethanol extracts components. The CD11a and CD11b antigens expressions on peripheral blood cells were analyzed by indirect immunofluorescence reaction at the age of 4, 8, 22 months. Statistical analysis was performed with the program Statistica 6.0.

Results. The development of genetically predisposed hepatocarcinomas in CBA male-mice was accompanied by a decrease in the expression of СD11a and CD11b antigens on blood cells. Dry multiphytoadaptogene extract administration during the first month of postnatal ontogenesis revealed the CD11a and CD11b antigens up-regulated expression. The effect of the dry multiphytoadaptogene extract is comparable to that for the liquid form of multifitoadaptogen previously identified.

Conclusion. Up-regulated LFA-1 and Mac-1 β₂ integrins expression on blood cells using dry multiphytoadaptogene extract administration during the first month of postnatal development is essential for enhance the contact interactions of immune effectors and cancer cells.

The study was performed in accordance with ethical principles adopted by the European Convention for the protection of vertebrate animals used for experimental and other scientific purposes.

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