IMMUNOLOGICAL PARAMETERS OF BONE MARROW IN NON-SMALL CELL LUNG CANCER
https://doi.org/10.17650/1726-9784-2019-19-2-47-54
Abstract
Introduction Generation of most immunocompetent cells takes place in bone marrow Bone marrow. As well, bone marrow is a peripheral lymphoid organ where antitumor effector cells and memory cells are present.
The aim of the work is to estimate peripheral lymphoid cell subpopulations in bone marrow of lung cancer patients.
Materials and methods Study has been done in 68 pts with lung cancer: squamous cell cancer (n = 28), adenocarcinoma (n = 38), other forms (n = 2). In all cases standard diagnostic and staging procedures were performed, as well as morphological (myelogram) and immunological study of bone marrow lymphocyte subpopulations. Multicolor Flow cytomtry was used for study of bone marrow lymphocyte populations. We studied T-cells and its subpopulations, B-cells, NK-cells, perforin-positive cells, and CD27-positive cells.
Results Squamous cell lung cancer was characterized by higher content of bone marrow mature T-cells (CD3), and CD8 lymphocytes. More typical for adenocarcinoma was mature B-cell reaction (CD20). Effector (perforin-positive) populations of lymphocytes also were related to histological type of cancer: for adenocarcinoma presence of CD4-positive cytotoxic lymphocytes and CD27-expression on effector cells. Perforin-containing lymphoid cells were in opposite correlation to erythrocaryocytes.
Conclusion Subpopulational lymphocyte content of bone marrow is related to histological variant of cancer and erythropoiesis in lung cancer patients.
About the Authors
N. N. TupitsynRussian Federation
24 Kashirskoe Shosse, Moscow 115478
T. M. Dzhumanazarov
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
A. D. Palladina
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
A. K. Alakhverdiyev
Russian Federation
86 Shosse Entuziastov, Moscow 111123
Build 1, 2/1 Barricadnaya St., Moscow 125993
S. V. Chulkova
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
1 Ostrovitianov St., Moscow 117997
P. V. Kononets
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
References
1. Steven A., Fischer S.A., Robinson B.W. Immunotherapy for lung cancer. Respirology 2016;21:821–33. DOI: 10.1111/resp.12789.
2. Buchan S.L., Anne Rogel A., AlShamkhani A. The immunobiology of CD27 and OX40 and their potential as targets for cancer immunotherapy. Blood 2018;131(1):39–48. DOI: 10.1182/blood-2017-07-741025.
3. Buchan S., Manzo T., Flutter B. et al. OX40- and CD27-mediated costimulation synergizes with antiPD-L1 blockade by forcing exhausted CD8+ T cells to exit quiescence. J Immunol 2015;194(1):125–33. DOI: 10.4049/jimmunol.1401644.
4. Takeuchi A., Saito T. CD4 CTL a cytotoxic subset of CD4+ T-cells, their differentiation and function. Front Immunol 2017;8:194. DOI: 10.3389/fimmu.2017.00194.
5. Mahnke Y.D., Brodie T.M., Sallusto F. et al. Who’s who of T-cell differentiation: human memory T-cell subsets. Eur J Immunol 2013;43(11):2797–809. DOI: 10.1002/eji.201343751.
6. Тупицын Н.Н., Зейналова П.А., Френкель М.А. и др. Роль костного мозга в прогнозе диффузной В-крупноклеточной лимфомы. Вопросы биологической, медицинской и фармацевтической химии 2015;4:40–7. [Tupitsyn N.N., Zeynalova P.A., Frenkel M.A. et al. The role of bone marrow in the prognosis of diffuse B-large cell lymphoma. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii = Problems of Biological, Medical and Pharmaceutical Chemistry 2015;4:40–7. (In Russ.)].
7. Тупицын Н.Н., Фалалеева Н.А., Моженкова А.В. и др. Роль костного мозга в прогнозе фолликулярной лимфомы. Российский биотерапевтический журнал 2016;15(3):99–102. DOI: 10.17650/1726-9784-2016-15-3-99-102. [Tupitsyn N.N., Falaleeva N.A., Mozhenkova A.V. et al. The role of bone marrow in the prognosis of follicular lymphoma. Rossiyskiy bioterapevticheskiy zhurnal = Russian Biotherapeutic Journal 2016;15(3):99–102. (In Russ.)].
8. Фалалеева Н.А., Моженкова А.В., Османов Е.А. и др. Особенности эритропоэза больных фолликулярной лимфомой. Вестник ФГБУ «РОНЦ им. Н.Н. Блохина» 2016;(3):35–8. [Falaleeva, N.A., Mozhenkova A.V., Osmanov E.A. et al. Features of erythropoiesis of patients with follicular lymphoma. Vestnik FGBU “RONTS im. N.N. Blokhina” = Journal of N.N. Blokhin Russian Cancer Research Center 2016;(3):35–8. (In Russ.)].
9. Тупицын Н.Н., Чэн Ц., Зейналова П.А. Иммунофенотипическое изучение дифференцировки эритрокариоцитов костного мозга у больных диффузной В-крупноклеточной лимфомой. Российский биотерапевтический журнал 2018;17(4):52–7. DOI: 10.17650/1726-9784-2018-17-4-52-57. [Tupitsyn N.N., Jiao C., Zeynalova P.A. Immunophenotypic study of bone marrow erythrokaryocyte differentiation in patients with diffuse large B-cell lymphoma. Rossiyskiy bioterapevticheskiy zhurnal = Russian Journal of Biotherapy 2018;17(4):52–7. (In Russ.)].
10. Chernysheva O., Markina I., Demidov L. et al. Bone marrow involvement in melanoma. Potentials for detection of disseminated tumor cells and characterization of their subsets by flow cytometry. Cells 2019;8(6):627. DOI: 10.3390/cells8060627.
11. Tupitsyn N.N., Mkrtchan V.A., Palladina A.D. et al. Bone marrow lymphocyte populations of innate immunity in breast cancer patients. GJMR 2020;20(2):20–7. DOI: 10.34257/GJMRFVOL20IS2PG21.
Review
For citations:
Tupitsyn N.N., Dzhumanazarov T.M., Palladina A.D., Alakhverdiyev A.K., Chulkova S.V., Kononets P.V. IMMUNOLOGICAL PARAMETERS OF BONE MARROW IN NON-SMALL CELL LUNG CANCER. Russian Journal of Biotherapy. 2020;19(2):47-54. (In Russ.) https://doi.org/10.17650/1726-9784-2019-19-2-47-54