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Russian Journal of Biotherapy

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Study of biocompatibility in vitro of ultrafine-grained Zn-based bioresorbable alloys

https://doi.org/10.17650/1726-9784-2022-21-3-40-49

Abstract

Background. Zinc alloys have advantages for use as biodegradable implantable orthopedic metal structures due to the absence of gas formation in comparison with magnesium alloys. But their mechanical properties are often has lower values.
Aim. Investigation of effect of high-pressure torsion (HPT) on strength, ductility, corrosion resistance, antimicrobial properties, surface cell colonization and biocompatibility of Zn-based alloys.
Materials and methods. The alloys of the Zn-x%Mg system (where x = 0; 1 and 1.7 %) in the initial undeformed state and after HPT were investigated in this work. Mechanical properties were studied on an Instron 3382 testing machine at room temperature. The biocompatibility of the alloys was evaluated by hemolytic activity and cytotoxicity assesment. We also studied the stimulation of colonization of the surface of the samples by mesenchymal multipotent stromal cells, as well as the presence of antimicrobial properties relative to the Escherichia coli culture. To study the degradation rate, the alloy samples were incubated in a standard nutrient medium for 8 days, assessing the change in their mass relative to the initial value.
Results. It has been established that HPT leads to an increase in the strength of pure Zn 2 times, and of Zn-1%Mg and Zn-1.7%Mg alloys by 3 and 5.5 times, respectively, with an increase in their ductility. At the same time, deformation treatment has practically no effect on the corrosion resistance of the initial materials. No significant increase in the hemolytic activity and bactericidal activity of the alloys was revealed during studies. However, a significant decrease in the ability of cells to colonize the surface of pure zinc was observed after HPT.
Conclusion. HPT leads to a significant increase in the strength and ductility of studied materials. At the same time, a decrease in the biocompatibility of zinc-based alloys after HPT did not observed. It was found that the discovered cytotoxic effect was obviously caused not so much by the alloy processing method as by its chemical composition. This makes it possible to evaluate the studied alloys of the Zn-x%Mg system treated by HPT (and, in particular, the Zn-1.7%Mg alloy) as a promising structure for the development of biodegradable orthopedic products.

About the Authors

N. S. Martynenko
A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences
Russian Federation

Natalia Sergeevna Martynenko

49 Leninsky Ave., Moscow 119334



N. Yu. Anisimova
A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences; N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia; National University of Science and Technology «MISIS»
Russian Federation

49 Leninsky Ave., Moscow 119334

24 Kashirskoe Shosse, Moscow 115522

4 Leninsky Ave., Moscow 119991

 



M. V. Kiselevskiy
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia; National University of Science and Technology «MISIS»
Russian Federation

24 Kashirskoe Shosse, Moscow 115522

4 Leninsky Ave., Moscow 119991

 



O. V. Rybalchenko
A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences
Russian Federation

49 Leninsky Ave., Moscow 119334



D. R. Temralieva
A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences; National University of Science and Technology «MISIS»
Russian Federation

49 Leninsky Ave., Moscow 119334

4 Leninsky Ave., Moscow 119991



D. V. Prosvirnin
A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences
Russian Federation

49 Leninsky Ave., Moscow 119334



S. V. Pivovarchik
A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences
Russian Federation


D. V. Filonenko
General Oncology Department of the A.S. Loginov Moscow Clinical Scientific Center, Moscow Healthcare Department
Russian Federation

86 Entuziastov Shosse, Moscow 111123



S. V. Dobatkin
A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences; National University of Science and Technology «MISIS»
Russian Federation

49 Leninsky Ave., Moscow 119334

4 Leninsky Ave., Moscow 119991



References

1. Korotkih N.G., Stepanov I.V., Larina O.E. et al. Application of mini-plates made of titan with nano-structural hydroxiapatite in complex treatment of lower jaw fractures. Vestnik novykh meditsinskikh tekhnologiy = Journal of New Medical Technologies 2011;18(2):356–7. (In Russ.).

2. Medvedev Yu.A., Shamanaeva L.S., Petruk P.S. et al. Nickelid titanium mesh for orbital floor reconstruction. Stomatologiya = Stomatology 2014;93(3):35–8. (In Russ.).

3. Xiao F., Zong C., Wang W. et al. Low-temperature fabrication of titania layer on 3D-printed 316L stainless steel for enhancing biocompatibility. Surf Coat Technol 2019;367:91–9. DOI: 10.1016/j.surfcoat.2019.03.071

4. Pogrel M.A. The concept of stress shielding in nonvascularized bone grafts of the mandible – a review of 2 cases. J Oral Maxillofac Surg 2021;79(1):266.e1–266.e5. DOI: 10.1016/j.joms.2020.09.024

5. Han H.-S., Loffredo S., Jun I. et al. Current status and outlook on the clinical translation of biodegradable metals. Mater Today 2019;23:57–71. DOI: 10.1016/j.mattod.2018.05.018

6. Xia D., Yang F., Zheng Y. et al. Research status of biodegradable metals designed for oral and maxillofacial applications: a review. Bioact Mater 2021;6(11):4186–208. DOI: 10.1016/j.bioactmat.2021.01.011

7. Tsakiris V., Tardei C., Clicinschi F.M. Biodegradable Mg alloys for orthopedic implants – a review. J Magnes Alloys 2021;9(6):1884–905. DOI: 10.1016/j.jma.2021.06.024

8. Kiselevsky M.V., Anisimova N.Yu., Polotsky B.Е. et al. Biodegradable magnesium alloys as promising materials for medical applications (review). Sovremenniye tekhnologii v meditsine = Modern Technologies in Medicine 2019;11(3): 146–57. (In Russ.). DOI: 10.17691/stm2019.11.3.18

9. Hänzi A.C., Gerber I., Schinhammer M. et al. On the in vitro and in vivo degradation performance and biological response of new biodegradable Mg–Y–Zn alloys. Acta Biomater 2010;6(5):1824–33. DOI: 10.1016/j.actbio.2009.10.008

10. Anisimova N., Kiselevskiy M., Martynenko N. et al. Anti-tumour activity of Mg-6%Ag and Mg-10%Gd alloys in mice with inoculated melanoma. Mater Sci Eng C 2021;130:112464. DOI: 10.1016/j.msec.2021.112464

11. Kabir H., Munir K., Wen C., Li Y. Recent research and progress of biodegradable zinc alloys and composites for biomedical applications: biomechanical and biocorrosion perspectives. Bioact Mater 2021;6(3):836–79. DOI: 10.1016/j.bioactmat.2020.09.013

12. Venezuela J., Dargusch M.S. The influence of alloying and fabrication techniques on the mechanical properties, biodegradability and biocompatibility of zinc: a comprehensive review. Acta Biomater 2019;87:1–40. DOI: 10.1016/j.actbio.2019.01.035

13. Li G., Yang H., Zheng Y. et al. Challenges in the use of zinc and its alloys as biodegradable metals: perspective from biomechanical compatibility. Acta Biomater 2019;97:23–45. DOI: 10.1016/j.actbio.2019.07.038

14. Chen Y., Huang P., Chen H. et al. Assessment of the biocompatibility and biological effects of biodegradable pure zinc material in the colorectum. ACS Biomater Sci Eng 2018;4(12):4095–103. DOI: 10.1021/acsbiomaterials.8b00957

15. Xiao C., Shi X.Y., Yu W.T. et al. In vivo biocompatibility evaluation of Zn-0.05Mg-(0, 0.5, 1wt%)Ag implants in New Zealand rabbits. Mater Sci Eng C 2021;119:111435. DOI: 10.1016/j.msec.2020.111435

16. Xiao C., Wang L., Ren Y. et al. Indirectly extruded biodegradable Zn-0.05wt%Mg alloy with improved strength and ductility: in vitro and in vivo studies. J Mater Sci Technol 2018;34(9): 1618–27. DOI: 10.1016/j.jmst.2018.01.006

17. Shao X., Wang X., Xu F. et al. In vivo biocompatibility and degradability of a Zn–Mg–Fe alloy osteosynthesis system. Bioact Mater 2022;7:154–66. DOI: 10.1016/j.bioactmat.2021.05.012

18. Bednarczyk W., Kawałko J., Wątroba M. et al. Microstructure and mechanical properties of a Zn-0.5Cu alloy processed by high-pressure torsion. Mater Sci Engi A 2020;776:139047. DOI: 10.1016/j.msea.2020.139047

19. Srinivasarao B., Zhilyaev A.P., Langdon T.G., Pérez-Prado M.T. On the relation between the microstructure and the mechanical behavior of pure Zn processed by high pressure torsion. Mater Sci Eng A 2013;562:196–202. DOI: 10.1016/j.msea.2012.11.027

20. Hernández-Escobar D., Unocic R.R., Kawasaki M., Boehlert C.J. High-pressure torsion processing of Zn–3Mg alloy and its hybrid counterpart: a comparative study. J Alloys Compd 2020;831:154891. DOI: 10.1016/j.jallcom.2020.154891

21. Rybalchenko O.V., Anisimova N.Yu., Kiselevsky M.V. et al. Effect of equal-channel angular pressing on structure and properties of Fe-Mn-С alloys for biomedical applications. Mater Today Commun 2022;30:103048. DOI: 10.1016/j.mtcomm.2021.103048

22. Choudhary R., Venkatraman S.K., Bulygina I. et al. Biomineralization, dissolution and cellular studies of silicate bioceramics prepared from eggshell and rice husk. Mater Sci Eng C 2021;118:111456. DOI: 10.1016/j.msec.2020.111456

23. ASTM G1-03-E. Standard practice for preparing, cleaning, and evaluating corrosion test specimens. West Conshohocken, PA: ASTM International, 2011.

24. Masters E.A., Ricciardi B.F., Bentley K.L.M. et al. Skeletal infections: microbial pathogenesis, immunity and clinical management. Nat Rev Microbiol 2022;20(7):385–400. DOI: 10.1038/s41579-022-00686-0

25. Bednarczyk W., Kawałko J., Rutkowski B. et al. Abnormal grain growth in a Zn-0.8Ag alloy after processing by high-pressure torsion. Acta Mater 2021;207:116667. DOI: 10.1016/j.actamat.2021.116667

26. Ren K., Zhang K., Zhang Y. et al. Effect of ECAP temperature on formation of triple heterogeneous microstructure and mechanical properties of Zn–1Cu alloy. Mater Sci Eng A 2021;826:141990. DOI: 10.1016/j.msea.2021.141990

27. García-Mintegui C., Córdoba L.C., Buxadera-Palomero J. et al. Zn-Mg and Zn-Cu alloys for stenting applications: from nanoscale mechanical characterization to in vitro degradation and biocompatibility. Bioact Mater 2021;6(12):4430–46. DOI: 10.1016/j.bioactmat.2021.04.015

28. Retegi-Carrión S., Ferrandez-Montero A., Eguiluz A. et al. The effect of Ca2+ and Mg2+ ions loaded at degradable PLA membranes on the proliferation and osteoinduction of MSCs. Polymers (Basel) 2022;14(12):2422. DOI: 10.3390/polym14122422

29. Li D., Zhang D. Yuan Q. et al. In vitro and in vivo assessment of the effect of biodegradable magnesium alloys on osteogenesis. Acta Biomater 2022;141:454–65. DOI: 10.1016/j.actbio.2021.12.032


Review

For citations:


Martynenko N.S., Anisimova N.Yu., Kiselevskiy M.V., Rybalchenko O.V., Temralieva D.R., Prosvirnin D.V., Pivovarchik S.V., Filonenko D.V., Dobatkin S.V. Study of biocompatibility in vitro of ultrafine-grained Zn-based bioresorbable alloys. Russian Journal of Biotherapy. 2022;21(3):40-49. (In Russ.) https://doi.org/10.17650/1726-9784-2022-21-3-40-49

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ISSN 1726-9784 (Print)
ISSN 1726-9792 (Online)