Development of the composition and characteristics of a wound-healing gel with L-arginine

Background. Currently, the issues of skin and mucous membrane wound therapy are becoming increasingly clear. One of the most modern and popular dosage forms of wound healing agents is a gel, which has undeniable pharmacological and technological advantages. L-arginine, which is a substrate for nitric oxide (II) (NO (II) synthases), which are involved in the formation of NO (II), was used as the main active ingredient in the developed dosage form.

Aim. Selection of the optimal gelling agent and development of the composition of a soft dosage form used for healing wounds of the skin and mucous membranes.

Materials and methods. Three model samples of gels made using potentially promising gelling agents were selected as objects of study: carbopol 940, sodium carboxymethyl cellulose, methyl cellulose. Rheological characteristics of the samples were studied by rotational viscometry on a Fungilab Premium viscometer (Spain) using a “cylinder in cylinder” measuring system. To study the biodegradation of the model sample, a FADT-1202A dissolution tester (FOCS Co., Ltd, China) equipped with a paddle stirrer was used. The degree of arginine release from the samples was estimated by the Kruvchinsky equilibrium dialysis method.

Results. As a result of studying the structural and mechanical properties of the model samples, it was revealed that methylcellulose in combination with L-arginine allows creating a gel composition with the greatest stability. when studying the biodegradation of the methylcellulose-based composition, it was found that the gel is capable of being on the oral mucosa for no more than 60 minutes. Then, using the Kruvchinsky equilibrium dialysis method during this period of time, the nature of the L-arginine release from the developed composition was established.

Conclusion. The conducted studies substantiated the choice of the wound-healing gel base. Based on the obtained results, methylcellulose was chosen as the optimal gelling agent for the production of a soft medicinal form with a wound-healing effect.

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