level of estrogen receptor α and β expression and coexpression in non-small cell lung cancer tissue
https://doi.org/10.17650/1726-9784-2018-17-4-111-114
Abstract
Objectives. Quantitative assessment of the expression and co-expression levels of estrogen receptors of different types (ERα and ERβ) in malignant tissues of large cohort of patients with non-small cell lung cancer (NSCLC).
Materials and methods. Measurement of ERα and ERβ expression levels was carried out on 167 malignant tissue samples by immunofluorescence flow cytometric method. Level of the ERα and ERβ expression was calculated as a ratio of specifically fluorescent cells (%) compared to the control (incubated with the secondary antibody only).
Results. The expression of ERα and ERβ was detected in all the NSCLC samples investigated. High variability in the level of the marker expression was revealed and it was shown 1,5–2,0-fold higher ERβ expression in the most tumors than that for ERα. The Spearman rank correlation coefficient of 0,364 (p <0,001) and the Pearson correlation coefficient of 0,401 (p <0,001) indicate statistically significant weak positive correlation between the levels of expression of ERβ and ERα.
Conclusion. The level of expression of ERβ as a main subtype of ER in NSCLC tissue could prove useful for selecting patients for antiestrogen therapyAbout the Authors
T. A. Bogush
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
O. M. Ryabinina
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
M. I. Papulina
M.V. Lomonosov Moscow State University
Russian Federation
1 bldg., 27 Lomonosovsky pr-t, Moscow 119991
Russian Federation
1 bldg., 27 Lomonosovsky pr-t, Moscow 119991
E. A. Bogush
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
A. N. Grishanina
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
A. V. Karpukhin
Research Centre for Medical Genetics
Russian Federation
1 Moskvorechie St., Moscow 115478
Russian Federation
1 Moskvorechie St., Moscow 115478
B. E. Polotsky
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Russian Federation
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
M. M. Davydov
N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
Russian Federation
24 Kashirskoe Shosse, Moscow 115478
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For citations:
Bogush T.A., Ryabinina O.M., Papulina M.I., Bogush E.A., Grishanina A.N., Karpukhin A.V., Polotsky B.E., Davydov M.M. level of estrogen receptor α and β expression and coexpression in non-small cell lung cancer tissue. Russian Journal of Biotherapy. 2018;17(4):111-114. (In Russ.) https://doi.org/10.17650/1726-9784-2018-17-4-111-114
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