Development of a novel monoclonal antibody against chitinase-like protein YKL-39 applicable for immunohistochemistry
https://doi.org/10.17650/1726-9784-2019-18-2-27-31
Abstract
Introduction. Mammalian chitinase-like proteins are produced in the areas of inflammation and in tumors. Some members of chitinase-like proteins family are studied as potential biomarkers of tumors (glioma, prostate and ovary). YKL-39 also known as chitinase-3-like 2 (CHI3L2) is a secreted protein produced by chondrocytes. Its high expression is also found in synoviocytes, lung heart and macrophages.
The aim of this study was the development of highly specific monoclonal antibodies against human YKL-39.
Materials and methods. Using recombinant full-length human YKL-39 as immunogen using hybridoma technology we have generated monoclonal antibody 1B2G4, that specifically binds YKL-39 in ELISA.
Results and conclusion. Obtained antibody was successfully tested in Western blot, immunocytochemistry, immunofluorescence and immunohistochemistry on FFPE sections. It was shown that the antibody binds the full-length YKL-39 protein and does not interact with other chitinase-like proteins.
Supporting agencies: The study was performed with the support from the Russian Foundation for Basic Research (grant No. 17-04-01857), Foundation for Development of the New Technologies Development and Commercialization Center “Skolkovo”.
About the Authors
A. N. Gratchev
N.N. Blokhin National Medical Research Center of Oncology Ministry of Health of Russia.
Russian Federation
24 Kashirskoe Sh., Moscow 115478.
Russian Federation
24 Kashirskoe Sh., Moscow 115478.
D. V. Samoilova
N.N. Blokhin National Medical Research Center of Oncology Ministry of Health of Russia.
Russian Federation
24 Kashirskoe Sh., Moscow 115478.
Russian Federation
24 Kashirskoe Sh., Moscow 115478.
S. N. Kurochkin
PrimeBioMed.
Russian Federation
Build. 20, 3 Nauchnij Proezd, Moscow 117246.
Russian Federation
Build. 20, 3 Nauchnij Proezd, Moscow 117246.
O. V. Kovaleva
N.N. Blokhin National Medical Research Center of Oncology Ministry of Health of Russia.
Russian Federation
24 Kashirskoe Sh., Moscow 115478.
Russian Federation
24 Kashirskoe Sh., Moscow 115478.
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Review
For citations:
Gratchev A.N., Samoilova D.V., Kurochkin S.N., Kovaleva O.V. Development of a novel monoclonal antibody against chitinase-like protein YKL-39 applicable for immunohistochemistry. Russian Journal of Biotherapy. 2019;18(2):27-31. (In Russ.) https://doi.org/10.17650/1726-9784-2019-18-2-27-31
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