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LYMPHOCYTE SUBSETS IN PERIPHERAL BLOOD OF HER2-POSITIVE AND TRIPLE NEGATIVE BREAST CANCER PATIENTS

https://doi.org/10.17650/1726-9784-2015-14-2-47-52

Abstract

The aim of this study was to investigate the relationship between the original amount of basic lymphocyte subpopulations in peripheral blood and the results of therapy in patients with HER2+ and triple negative breast cancer. Before treatment was conducted immunophenotyping of peripheral blood lymphocytes by flow cytometry using a panel of monoclonal antibodies to surface markers and intracellular lymphocyte antigen FOXP3 and determining the cytotoxic activity of NK-cells. Cytotoxic activity was determined with MTT colorimetric test against K-562 cells. Revealed some differences in the composition of the population of lymphocytes and the number of regulatory T cells in patients with HER2+ and triple negative breast cancer (BC). Discuss the relationship between the number of suppressor cells and the degree of therapeutic pathomorphosis of tumor.

About the Authors

A. I. Chertkova
ФГБНУ «Российский онкологический научный центр им. Н.Н.Блохина»
Russian Federation


E. G. Slavina
ФГБНУ «Российский онкологический научный центр им. Н.Н.Блохина»
Russian Federation


L. G. Zhukova
ФГБНУ «Российский онкологический научный центр им. Н.Н.Блохина»
Russian Federation


I. P. Ganshina
ФГБНУ «Российский онкологический научный центр им. Н.Н.Блохина»
Russian Federation


M. F. Okruzhnova
ФГБНУ «Российский онкологический научный центр им. Н.Н.Блохина»
Russian Federation


E. K. Shoua
ФГБНУ «Российский онкологический научный центр им. Н.Н.Блохина»
Russian Federation


V. A. Nurtdinova
ФГБНУ «Российский онкологический научный центр им. Н.Н.Блохина»
Russian Federation


A. A. Borunova
ФГБНУ «Российский онкологический научный центр им. Н.Н.Блохина»
Russian Federation


Z. G. Kadagidze
ФГБНУ «Российский онкологический научный центр им. Н.Н.Блохина»
Russian Federation


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Review

For citations:


Chertkova A.I., Slavina E.G., Zhukova L.G., Ganshina I.P., Okruzhnova M.F., Shoua E.K., Nurtdinova V.A., Borunova A.A., Kadagidze Z.G. LYMPHOCYTE SUBSETS IN PERIPHERAL BLOOD OF HER2-POSITIVE AND TRIPLE NEGATIVE BREAST CANCER PATIENTS. Russian Journal of Biotherapy. 2015;14(2):47-52. (In Russ.) https://doi.org/10.17650/1726-9784-2015-14-2-47-52

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ISSN 1726-9784 (Print)
ISSN 1726-9792 (Online)