INHIBITION OF MT1-MMP (MMP14) IMPROVES ANT-GLIOMA EFFECT OF COMBINATION: TEMOZOLOMIDE-IONIZING RADIATION IN THE GLIOBLASTOMA CANCER CELLS
https://doi.org/10.17650/1726-9784-2015-14-2-53-58
Abstract
The main goal of our study is to provide clinical and biological assessment of MMP14 as gene that causes recurrent glioblastoma. We analyzed expression of MMP14 in 108 and 129 samples represented by Rembrandt 8РOncomine databases. The biological significance was done is based on genetic knockdown and rescuing of MMP14 attenuated U87 and U251 glioma cells followed by cytofluorimetry and array gene expression. As expected, genetic silencing of MMP14 led to accumulation cells in the “G2” phase of cell divisions which correlates to cell kinase’s expression. Furthermore, exposure of MMP14 to ionizing radiation and temozolomidt treatments prolongs glioma cell proliferation and increase the anti-glioma effect of temozolomide-ionizing radiation therapeutic combination. Our data suggest that most of the neoplastic glioma cells express significantly MMP14.Therefore, alteration of MMP14, improves anti-tumor effect.
About the Authors
I. V. Ulasov
Центр опухолей головного мозга, Шведский медицинский центр; ФГБНУ «РОНЦ им Н.Н. Блохина»
Russian Federation
Russian Federation
N. V. Kaverina
ФГБНУ «РОНЦ им Н.Н. Блохина»
Russian Federation
Russian Federation
Z. G. Kadagidze
ФГБНУ «РОНЦ им Н.Н. Блохина»
Russian Federation
Russian Federation
A. Y. Baryshnikov
ФГБНУ «РОНЦ им Н.Н. Блохина»
Russian Federation
Russian Federation
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Review
For citations:
Ulasov I.V., Kaverina N.V., Kadagidze Z.G., Baryshnikov A.Y. INHIBITION OF MT1-MMP (MMP14) IMPROVES ANT-GLIOMA EFFECT OF COMBINATION: TEMOZOLOMIDE-IONIZING RADIATION IN THE GLIOBLASTOMA CANCER CELLS. Russian Journal of Biotherapy. 2015;14(2):53-58. (In Russ.) https://doi.org/10.17650/1726-9784-2015-14-2-53-58
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