THE CHARACTERISTIC OF BONE MARROW STEM CELLS OF PATIENTS WITH THE COMPLICATED DIABETES
https://doi.org/10.17650/1726-9784-2017-16-1-47-50
Abstract
Introduction. An increase in patients with diabetes and a large number of amputations of limbs make the search for new treatments for these patients. Materials and methods. The study included 17 patients with complicated forms of diabetes. Patients were studied in the bone marrow immediately after surgery - the amputation of the limb. The number of hematopoietic stem cells and mesenchymal stem cells using specific markers were determined in the laboratory. The immunohistochemical characteristics of bone marrow were carried out for all samples. Results and conclusion. The obtained results indicate the biological value of bone marrow removed with amputation of the lower limb of the patient with complicated diabetes mellitus. The ease of obtaining bone marrow makes the procedure approachable in surgical practice. The presence of hematopoietic and mesenchymal stem cells provides the opportunity to use them in autologous cell therapy for patient.
About the Authors
L. P. Nikolaeva
V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Ministry of Health of Russia
Russian Federation
Russian Federation
D. V. Cherdantsev
V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Ministry of Health of Russia
Russian Federation
Russian Federation
K. S. Titov
Moscow Clinical Research Center of Health Department of Moscow; N.I. Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
References
1. Николаева Л.П., Черданцев Д.В., Степаненко А.В., Дятлов В.Ю. Изменение состояния антиоксидантной системы у больных с синдромом диабетической стопы. Фундаментальные исследования 2010; 11: 95-7.
2. Питерс-Хармел Э., Матур Р. Сахарный диабет: диагностика и лечение. М.: ИД «Практика», 2008. 496 с.
3. Barc P., Skora J., Pupka A. Bone-marrow cells in therapy of critical limb ischaemia of lower extremities - own experience. Acta Angiolog 2006; 12(4): 155-66.
4. Пальцев М.А. Медицина XXI века в свете клеточной биологии. Вестн. РАМН 2004; 9: 3-11.
5. Norgren L., Hiatt W.R., Dormandy J.A. et al. Inter-society consensus for the management of peripheral arterial disease (TASC II). J Vasc Surg 2007; 45 (suppl S): S5-67. DOI: 10.1016/j. jvs. 2006.12.037. PMID: 17223489.
6. Bruder S.P., Fink D.J., Caplan A.I. Mesenchymal stem cells in bone development, bone repair, and skeletal regeneration therapy. J Cell Biochem 1994; 56: 283-94. DOI: 10.1002/jcb.240560303. PMID: 7876320.
7. Gu Y.Q., Zhang J., Guo L.R. et al. Transplantation of autologous bone marrow mononuclear cells for patients with lower limb ischemia. Chin Med J 2008; 121(11): 963-7. PMID: 18706241.
8. Weaver C.V., Garry D.G. Regenerative biology: a historical perspective and modern applications. Regen Med 2008; 3: 63-82. DOI: 10.2217/17460751.3.1.63. PMID: 18154463.
9. Krause J. Bone marrow overview. In: Rodak B., ed. Hematology: Clinical Procedures and Applications. 2nd ed. Philadelphia: WB Saunders, 2002. P. 188-195.
10. Сисла Б. Руководство по лабораторной гематологии. Пер. с англ. Под общ. ред. А.И. Воробьева. М.: Практическая медицина, 2011. 352 c.
11. Jamshidi K., Swaim W. R. Bone marrow biopsy with unaltered architecture: A new biopsy device. J Lab Clin Med 1971; 77: 335. PMID: 5540776.
12. Shake J.G., Gruber P.J., Baumgartner W.A. et al. Mesenchimal stem cell implantation in a swine myocardial infarct model: engraftment and functional effects. Ann Thorac Surg 2002; 73: 1919-56. PMID: 12078791.
13. Lu D., Chen B., Liang Z. et al. Comparison of bone marrow mesenchymal stem cells with bone marrow-derived mononuclear cells for treatment of diabetic critical limb ischemia and foot ulcer: A double-blind, randomized, controlled trial. Diabetes Res Clin Pract 2011; 92(1): 26-36. DOI: 10.1016/j.diabres.2010.12.010. PMID: 21216483.
14. Packham D.K., Fraser I.R., Kerr P.G., Segal K.R. Allogeneic Mesenchymal Precursor Cells (MPC) in Diabetic Nephropathy: A Randomized, Placebocontrolled, Dose Escalation Study. EBioMedicine 2016; 9: 1-7. DOI: 10.1016/j.ebiom.2016.09.011. PMID: 27743903. PMCID: PMC5078602.
Review
For citations:
Nikolaeva L.P., Cherdantsev D.V., Titov K.S. THE CHARACTERISTIC OF BONE MARROW STEM CELLS OF PATIENTS WITH THE COMPLICATED DIABETES. Russian Journal of Biotherapy. 2017;16(1):47-50. (In Russ.) https://doi.org/10.17650/1726-9784-2017-16-1-47-50
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